Method for the isolation of Escherichia coli relaxed mutants, utilizing near-ultraviolet irradiation
نویسندگان
چکیده
منابع مشابه
Isolation of "relaxed" mutants of Escherichia coli.
All previously described mutants showing relaxed control of ribonucleic acid (RNA) synthesis (RCrel strains) have been derived from a common ancestor, Escherichia coli strain W6 (L. Alfoildi, G. S. Stent, and R. C. Clowes, J. Mol. Biol. 5:348, 1962), first described by E. Borek, A. Ryan, and I. Rockenbach (J. Bacteriol. 69:460, 1955). We wish to describe the isolation of independent relaxed mut...
متن کاملImproved Method for the Isolation of Thymine-requiring Mutants of Escherichia Coli.
Thymine-requiring mutants of Escherichia coli were rarely found before the observation by Okada, Yanagisawa, and Ryan (Z. Vererbungslehre 92:403, 1961) that cultures of bacteria grown in the presence of high concentrations of aminopterin, thymine, purines, and serine contain a surprising number of cells unable to synthesize their own supply of thymine. They showed that these thymine-dependent m...
متن کاملA method for isolation of mutants of Escherichia coli altered in their ability to metabolize glycogen.
Katz, J., Dunn, A., Chenoweth, M. &Golden, S . (1974b)Biochem. J. 142,171-183 Osmundsen, H. & Sherratt, H. S . A. (1975) FEBSLett. 55, 38-41 Popovic, V. & Popovic, P. (1960) J. Appl. Physiol. 15, 727-728 Sherratt, H. S . A. (1969) Br. Med. Bull. 25,256255 Sherratt, H. S. A. & Osmundsen, H. (1976) Biochem. Pharmacol. 25,743-750 Tanaka,K., Miller,E. M. &Isselbacher, K. J. (1971)Proc. Natl. Acad. ...
متن کاملEscherichia coli mutants induced by multi-ion irradiation
Wild-type Escherichia coli K12 strain W3110 was irradiated by 10 keV nitrogen ions. Specifically, irradiation was performed six times by N(+) ions, followed by the selection of lac constitutive mutants, and each time a stable S55 mutant was produced. By sequencing the whole genome, the fine map of S55 was completed. Compared with reference sequences, a total of eighteen single nucleotide polymo...
متن کاملIsolation of vitamin B 12 transport mutants of Escherichia coli.
Escherichia coli KBT001, a methionine-vitamin B(12) auxotroph, was found to require a minimum of 20 molecules of vitamin B(12) (CN-B(12)) per cell for aerobic growth in the absence of methionine. After mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine and penicillin selection, two kinds of B(12) transport mutant were isolated from this strain. Mutants of class I, such as KBT069, were defect...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1976
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.127.3.1587-1589.1976